Effects of carbendazim on in vitro micropropagation and flowering of pepper elder herbal plant.

E Mohan Raj¹, Teoh Lydia², Abdul Bakrudeen Ali Ahmed¹, Muneeb M Musthafa³, Vidya Devanathadesikan Seshadri⁴, Vijay Lobo¹, R Vidhyavathi¹, Faiz MMT Marikar⁵*

¹Department of Biochemistry, Centre for Research and Development (CRD), PRIST Deemed University, Vallam, Thanjavur, Tamil Nadu, India
²Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
³Department of Biosystems Technology, Faculty of Technology, South Eastern University of Sri Lanka, University Park, Oluvil, #32360, Sri Lanka
⁴Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdul Aziz University, Kingdom of Saudi Arabia
⁵General Sir John Kotelawala Defence University, Ratmalana, Sri Lanka

Abstract

The present study reports on application of carbendazim for micropropagation and in vitro flowering of Peperomia pellucida. In vitro multiplication of shoot tips and axillary nodal explants were used in 25-100 mgL^-1 carbendazim in 70% alcohol treatment for 5 min in MS (Murashige and Skoog) medium and MS hormone medium The explants were cultured on MS medium supplemented with auxins, cytokinins and additives (Casin hydrolyte and glutamine) used to proliferate the shoots. The maximum number of shoots (21.5/shoot tip) were developed on MS medium supplemented with 2.0 mgL^-1 KIN (Kinetin) 0.5 mgL^-1 NAA (α- Naphthalene acetic acid), 100 mgL^-1 casein hydrolysate and 100 mgL^-1 glutamine additives. The highest number of roots per shoot (17.6) and highest rooting frequency (94.2%) were obtained when the adventitious shoots were inoculated on MS medium with 1.5 mgL^-1 IAA (Indole-3-acetic acid). The highest number of shoot development (75.4 %) was achieved on MS medium supplemented with 2.0 mgL^-1 KIN 2.0 + 0.5 mgL^-1 ABA (Abscisic acid) with 5% sucrose. It was found that MS medium devoid of 1.5 mgL^-1 IAA promote the root formation and maturation of in vitro flower in P. pellucida. Acclimatized plantlets were transferred to botanical garden and the characters compared with mother plant of P. pellucida.

Keywords: Additives, flower bud, Plant growth regulators, Sucrose, Surface sterilization

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* - Corresponding Author

Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka

Copyright © 2007 by the Faculty of Agriculture, University of Ruhuna

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